PROJECT HELIX-HTT | SCF APC Cargo Atlas — Antigen-Presenting Cell Cargo Architecture

PROJECT HELIX-HTT | Neuro-Oncology Vaccine Program

1. Purpose

The APC Cargo Atlas defines what antigen-presenting cells must carry, process, display, and signal in order to convert HELIX-VAX antigens into a durable anti-tumor immune response.

APC = Antigen-Presenting Cell, mainly:

APC Type	Primary Role
Dendritic cells	Strongest T-cell priming
Macrophages / microglia	CNS immune interpretation
B cells	Secondary antigen presentation
Monocytes	Inflammatory bridge APCs

2. APC Cargo Categories

Cargo Class	Function	Vaccine Relevance
Antigen cargo	Tumor peptides / neoantigens	Defines immune target
HLA cargo	MHC-I / MHC-II complexes	Displays antigen
Co-stimulatory cargo	CD80, CD86, CD40	Activates T cells
Cytokine cargo	IL-12, IFN-I, TNF-α	Shapes immune response
Checkpoint cargo	PD-L1, CTLA4-related signals	May suppress response
Metabolic cargo	redox / hypoxia state	Determines APC fitness
Neuroimmune cargo	microglial activation signals	CNS safety control

3. APC Cargo Atlas Table

Cargo ID	Cargo Type	Key Markers	Desired State	SCF Interpretation
APC-C01	Antigen peptide cargo	VAMI antigens	High fidelity	Correct tumor target loading
APC-C02	MHC-I cargo	HLA-A/B/C, B2M	High	CD8 T-cell activation
APC-C03	MHC-II cargo	HLA-DR/DQ/DP	Functional	CD4 helper support
APC-C04	Co-stimulation	CD80, CD86, CD40	High	Immune activation enabled
APC-C05	Maturation cargo	CCR7, CD83	High	Lymph node trafficking
APC-C06	Cytokine cargo	IL-12, IFN-α/β	Balanced-high	Durable anti-tumor priming
APC-C07	Suppression cargo	PD-L1, IL-10, IDO1	Low	Avoid immune silencing
APC-C08	Redox cargo	GSH:GSSG, ROS load	Stable	APC metabolic competence
APC-C09	Hypoxia cargo	HIF-1α, CAIX	Low	Avoid hypoxia-impaired priming
APC-C10	Neuroimmune cargo	IBA1, TREM2, IL-1β	Balanced	CNS safety compatibility

4. APC Cargo Flow

Tumor Antigen Uptake
↓
Antigen Processing
↓
MHC-I / MHC-II Loading
↓
Co-Stimulatory Signal Expression
↓
Cytokine Programming
↓
T-Cell Priming
↓
Memory Formation
↓
Tumor Killing

5. SCF APC Cargo Quality Index

APC-CQI =
Antigen Loading Fidelity
+ MHC Presentation Strength
+ Co-Stimulation
+ Cytokine Support
+ Metabolic Fitness
+ Neuroimmune Compatibility
- Suppression Cargo
- Hypoxia Burden

Score	Interpretation
≥85	APC vaccine-ready
70–84	functional, optimize
55–69	weak priming risk
<55	not suitable

6. HELIX-VAX APC Cargo Requirements

For HELIX-VAX-01, APCs must demonstrate:

Requirement	Minimum Standard
Antigen uptake	detectable VAMI antigen processing
MHC-I presentation	strong CD8-compatible display
MHC-II presentation	helper T-cell support present
CD80/CD86	elevated after stimulation
IL-12 / IFN-I	present but not excessive
PD-L1 / IL-10 / IDO1	controlled
CNS safety	no excessive microglial inflammatory profile

7. APC Cargo Failure Modes

Failure Mode	Meaning	Correction
Poor antigen uptake	APC cannot internalize vaccine	optimize delivery platform
Weak MHC-I loading	weak CD8 activation	adjust construct/linkers
Low co-stimulation	T cells become anergic	improve adjuvant strategy
Excess PD-L1 / IL-10	immune suppression	add conditioning strategy
High ROS / hypoxia burden	metabolically weak APCs	RHENOVA correction
Hyperactive neuroimmune cargo	CNS inflammation risk	reduce adjuvant intensity

8. Output Files

File	Description
APC-01	APC Cargo Marker Panel
APC-02	Antigen Loading Report
APC-03	MHC Presentation Report
APC-04	Co-Stimulation Report
APC-05	Suppression Cargo Report
APC-06	Neuroimmune Safety Cargo Report

9. Status

APC Cargo Atlas: Complete

Next recommended deliverable: Dendritic Cell Activation Report (DV-04).